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All Journal Jurnal Pendidikan Kimia Indonesia SOSIALITAS (Jurnal Ilmiah Pend. Sos-Ant) Jurnal Penelitian Pendidikan Guru Sekolah Dasar Biosaintifika: Journal of Biology & Biology Education JFIOnline Jurnal Fisika dan Terapannya Pharmaceutical Sciences and Research (PSR)
Nurul Amalia
Jurusan Farmasi, FMIPA, Universitas Muhammadiyah
Agriculture, Biological Sciences & Forestry Chemistry Decision Sciences, Operations Research & Management Education Health Professions Medicine & Pharmacology Physics Social Sciences

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Journal : Biosaintifika: Journal of Biology

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In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D Budisantoso, Iman; Amalia, Nurul; Kamsinah, Kamsinah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11018

Abstract

The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of Vanda sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from Vanda sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.
Co-Authors . Sediarso, . Achmad Lutfi Aini, Nur Qurrotur Hadi Sunaryo Ihsan Ihsan Iman Budisantoso Kamsinah Kamsinah PANDU PAKSI, HENDRIK Rahmaniah, Rahmaniah Rukmana, Maissy Diana Sediarso Sediarso, Sediarso Umah, Putri Amiratul Vera Devani